Neural Tube
Neural Tube - Related Videos
12:48
Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
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Cited by 26 •
2012
Isolation of embryonic neural crest from the neural tube facilitates the use of in vitro methods for studying migration, self-renewal, and multipotency of neural crest.
07:37
Neural Tube Closure in Mouse Whole Embryo Culture
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Cited by 22 •
2011
A method allowing for direct pharmacological manipulation of mouse embryos during neurulation that bypasses maternal metabolism is described. The technique can be adapted to study different aspects of neurulation by varying the time point and pharmacological agent.
09:57
Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection
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Cited by 11 •
2015
Here we report dual labeling of neural crest cells and blood vessels using chickGFP neural tube intraspecies grafting combined with intra-vascular DiI injection. This experimental technique allows us to simultaneously visualize and study development of the NCC-derived (enteric) nervous system and the vascular system, during organogenesis.
07:55
Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation
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Cited by 12 •
2010
This video demonstrates how to visualize axonal pathways of genetically defined groups of neurons in the embryonic chick spinal cord utilizing in ovo electroporation of reporter genes under the control of specific enhancer elements.
12:41
In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations
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Cited by 27 •
2012
A method by which gene expression in the neural tube can be downregulated in a cell type-specific, traceable manner is described. We demonstrate how in ovo electroporation of microRNA-based plasmids that elicit spatiotemporally controlled RNA interference can be used to investigate commissural axon guidance in the developing neural tube.
FAQs
Signals during neurulation first influence a region of ectoderm, the outer embryonic tissue, to become the neural plate. This change establishes the tissue that will undergo later shaping rather than remaining ordinary ectoderm. Studying these signals helps researchers explain how early embryonic development initiates central nervous-system formation and how altered signaling could disrupt normal patterning.
The neural plate edges elevate, bend toward one another, and fuse in a coordinated sequence. These movements transform a flattened embryonic region into a continuous tube, creating the structural basis for later brain and spinal-cord development. Because closure is essential to this transition, disruptions during the process can be linked to neural tube defects such as spina bifida and anencephaly.
Neural crest cells separate from the developing neural structure and migrate to other locations in the embryo. Their behavior differs from the tissue that remains organized as the neural tube, making them important for understanding how neurulation generates both central nervous-system structures and migrating cell populations. Tracking their detachment and movement broadens analysis beyond tube closure alone.
A neurulation study can follow the sequence from ectodermal signaling and neural-plate formation through edge elevation, inward folding, and fusion. Researchers may then examine the detachment and migration of neural crest cells. Organizing observations in this order connects cellular changes with the larger developmental outcome and helps identify where abnormal development may arise.
Neural tube research provides a framework for examining how the vertebrate central nervous system is patterned during embryonic development. It connects early tissue shaping with the later formation of the brain and spinal cord. This makes the topic useful in developmental biology, where researchers seek to relate embryonic movements and signals to organized nervous-system formation.
Researchers use neurulation to investigate how developmental disruptions can produce neural tube defects, including spina bifida and anencephaly. The process therefore links embryonic biology with congenital-disease research and preventive strategies. Findings from this area also support attention to folate supplementation as an example of a prevention-focused approach associated with neural tube health.