Organelle Morphology
Organelle Morphology - Related Videos
07:29
A high-throughput method to globally study the organelle morphology in S. cerevisiae
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Cited by 2 •
2009
GFP-fusion proteins are widely used to visualize organelles by confocal microscopy. However, screening for mutations that affect the morphology of organelles generally requires individual mutagenesis and is time consuming. Here, we demonstrate a method to simultaneously incorporate organelle-GFP markers in almost 5,000 non-essential genes in yeast.
08:47
Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans
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Cited by 2 •
2019
This study outlines quantitative measurements of synaptic size and localization, muscle morphology, and mitochondrial shape in C. elegans using freely available image processing tools. This approach allows future studies in C. elegans to quantitatively compare the extent of tissue and organelle structural changes as a result of genetic mutations.
15:39
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
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Cited by 8 •
2019
Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.
01:43
The Movement of Organelles and Vesicles
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2023
In eukaryotic cells, cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
12:42
Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity
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Cited by 4 •
2012
A fundamental quest in cell biology is to define the mechanisms that underlie the identity of the organelles that make eukaryotic cells. Here we propose a method to identify the genes responsible for the morphological and functional integrity of plant organelles using fluorescence microscopy and next-generation sequencing tools.
FAQs
Organelle form reflects the combined effects of membrane behavior, protein complexes, and forces generated by the cytoskeleton. These factors can alter size, shape, and spatial arrangement rather than maintaining a fixed structure. Studying their interaction helps explain how organelles reorganize during normal cellular activity and how structural changes may accompany altered cellular function.
Fusion joins membrane-bound structures, whereas fission separates them into distinct compartments. Budding produces membrane-derived structures, and remodeling changes existing architecture without being limited to simple joining or division. Together, these processes continually reshape mitochondria, the endoplasmic reticulum, and the Golgi apparatus, allowing their organization to respond to cellular demands and changing conditions.
Transport pathways help connect organelle structure with the movement and organization of cellular materials. Changes in trafficking can be reflected in the architecture of compartments such as the endoplasmic reticulum and Golgi apparatus. Consequently, morphology provides a structural perspective on intracellular transport, while transport-related changes can help explain why an organelle becomes reorganized or dysfunctional.
Researchers examine organelle size, shape, structure, and spatial organization through microscopy, then use image-based analysis to characterize those features. Comparing observed patterns across cells or conditions can reveal remodeling, altered distribution, or structural irregularities. This approach converts visual information into evidence that can be related to metabolism, trafficking, division, stress, or disease.
Morphological changes can provide clues about cellular metabolism, intracellular trafficking, and cell division, as well as responses to stress. They may also indicate organelle dysfunction associated with disease. Because structure changes alongside cellular activity, morphology analysis helps researchers connect visible reorganization with functional states rather than treating organelles as isolated static compartments.
This analysis is useful when researchers need to investigate how cells develop, adapt, or respond to changing conditions. It also supports studies of disease mechanisms and organelle dysfunction by linking altered architecture with broader cellular processes. In biology, microscopy-based structural observations therefore complement functional investigations of metabolism, transport, division, and stress responses.