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Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans
JoVE Journal
Biology
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JoVE Journal Biology
Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans
DOI:

08:47 min

July 05, 2019

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Chapters

  • 00:04Title
  • 00:59Slide Preparation
  • 01:48Assessing Synaptic Morphology
  • 02:47Quantification of the Synaptic Region
  • 03:45Measurement of Muscle Cell Area
  • 04:36Segmentation and Quantification of Myosin Fiber Length
  • 06:39Results: Function of MEC-17 in the Nervous System
  • 08:14Conclusion

Summary

Automatic Translation

This study outlines quantitative measurements of synaptic size and localization, muscle morphology, and mitochondrial shape in C. elegans using freely available image processing tools. This approach allows future studies in C. elegans to quantitatively compare the extent of tissue and organelle structural changes as a result of genetic mutations.

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