Pericyte Astrocyte Co-culture
Pericyte Astrocyte Co-culture - Related Videos
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Isolating and Culturing Mouse Cerebral Pericytes
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This video demonstrates a method for isolating cerebral pericytes from a mouse brain. The isolated cells are cultured in pericyte-specific media to promote pericyte proliferation.
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Isolation and Culture of Primary Brain Pericytes: A Procedure to Isolate and Culture Pericytes from Bovine Brain Capillaries Without Endothelial Cell Contamination
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In this video, we demonstrate the procedure to isolate and culture pericytes from bovine brain capillaries without endothelial cell contamination.
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Isolation and Culture of Mouse Cortical Astrocytes
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Astrocytes have been recognized to be versatile cells participating in fundamental biological processes that are essential for normal brain development and function, and central nervous system repair. Here we present a rapid procedure to obtain pure mouse astrocyte cultures to study the biology of this major class of central nervous system cells.
02:35
Visualizing Intracellular Transport of Cargo in Cultured Astrocytes
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Source: Creighton, B. A., et al. Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes. J. Vis. Exp. (2019)This video demonstrates the visualization of intracellular cargo transport in cultured astrocytes using a fluorescent acidotropic probe that selectively labels acidic endolysosomal vesicles. Time-lapse imaging and Z-stack acquisition with a confocal microscope allow tracking of fluorescently labeled cargo throughout the three-dimensional cell volume.
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A Simple Technique to Isolate and Culture Murine Astrocytes
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2025
The video demonstrates a simple method to culture murine astrocytes in isolation from other brain cells. During culturing, by using astrocyte-specific media and inhibiting the adherence of non-astrocytic cells, the method selectively cultures astrocytes. These cultured astrocytes exhibit stellate morphology with fine processes.
FAQs
Two communication routes are central: direct cell contact and soluble factors released into the culture environment. Shared systems permit contact, whereas compartmentalized designs can separate the cells while preserving signaling through diffusible molecules. Comparing these arrangements helps distinguish contact-dependent effects from factor-mediated effects when assessing pericyte behavior, vascular stability, barrier properties, or inflammatory signaling.
Changes observed in one cell population can be interpreted as part of a reciprocal interaction rather than as an isolated cell response. Astrocytes can influence pericyte behavior, while pericytes can modify vascular stability, barrier properties, and inflammatory signaling associated with the culture. This bidirectional perspective is important for linking cellular communication to neurovascular function and disease-related changes.
Responses to injury or inflammation provide experimental conditions for examining how neurovascular interactions change. In this context, researchers can ask whether altered communication is associated with changes in pericyte behavior, vascular stability, barrier properties, or inflammatory signaling. Because the cells are studied together under controlled in vitro conditions, the model helps connect a stimulus with coordinated cellular outcomes.
A basic workflow is to establish the two cell populations either in a shared culture arrangement or in separate compartments, then examine their communication and resulting phenotypes. The chosen configuration determines whether direct contact is included. Measurements can focus on pericyte behavior, vascular stability, barrier properties, and inflammatory signaling, allowing the experimental design to match the mechanism under investigation.
Researchers would choose this model when they need a controlled platform for neurovascular unit questions that cannot be reduced to a single cell type. It is particularly relevant for studying blood-brain barrier regulation, neurovascular function, and cellular responses linked to injury or inflammation. The co-culture context preserves communication between vascular and neural cells while avoiding immediate dependence on an animal experiment.
Findings from the co-culture can reveal how interactions relate to barrier regulation, vascular stability, or inflammatory signaling and can support comparison of disease-related conditions. The system also offers a preliminary setting for evaluating potential therapeutic strategies before animal studies. Its value lies in connecting cellular mechanisms with measurable neurovascular outcomes under experimentally controlled conditions.