Spiral Ligament Removal
Spiral Ligament Removal - Related Videos
07:51
Spiral Ganglion Neuron Explant Culture and Electrophysiology on Multi Electrode Arrays
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Cited by 5 •
2016
We present a protocol to culture primary murine spiral ganglion neuron explants on multi electrode arrays to study neuronal response profiles and optimize stimulation parameters. Such studies aim to improve the neuron-electrode interface of cochlear implants to benefit hearing in patients as well as the energy consumption of the device.
02:35
Culturing Murine Spiral Ganglion Neuron Explants on Multielectrode Arrays
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2025
This video demonstrates the process for isolating spiral ganglion explants from the inner ear and co-culturing them with the organ of Corti on a multielectrode array. Spiral ganglia are carefully extracted and sectioned into smaller tissue sections or explants. These explants are cultured on the multielectrode array's active area to promote neuronal process outgrowth.
03:51
Label-Free Neutrophil Separation from Tracheal Secretion Using Spiral Microfluidics Channel
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2025
This video demonstrates separating neutrophils from tracheal secretions using a microfluidic device with spiral channels. The device achieves efficient separation based on cell size by utilizing varying thicknesses and trapezoidal-shaped channels. The interplay between forces results in large neutrophils accumulating near the inner wall while erythrocytes and mucin aggregates remain near the outer wall, ensuring effective neutrophil separation.
02:40
Analyzing Neural Activity in Primary Murine Spiral Ganglion Neurons Using Multielectrode Arrays
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2025
This video demonstrates the recording of spontaneous and stimulation-dependent neural activity from primary murine spiral ganglion neuron (SGN) cultures on a multielectrode array (MEA). It involves washing and preparing the MEA, recording spontaneous neural activity, and applying controlled electrical stimuli to identify stimulation-dependent neuronal activity.
08:03
Treatment of Ligament Constructs with Exercise-conditioned Serum: A Translational Tissue Engineering Model
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Cited by 7 •
2017
We present a model of ligament tissue in which three-dimensional constructs are treated with the human exercise-conditioned serum and analyzed for collagen content, function, and cellular biochemistry.
FAQs
Preserving adjacent structures maintains the anatomical context needed to interpret the isolated tissue accurately. Damage to the stria vascularis or sensory epithelium could compromise studies of cochlear architecture and obscure relationships involved in ion regulation and auditory function. Careful separation therefore improves the biological value of subsequent structural, immunolabeling, or molecular analyses.
The preparation can support examination of cochlear architecture, ion regulation, and mechanisms associated with hearing. Histology reveals tissue organization, immunolabeling can identify selected biological components, and molecular analysis can investigate tissue-associated molecular features. Using these approaches together helps connect the ligament’s structure with inner-ear development, auditory function, or pathology.
Microscopy provides the visual control required to distinguish the spiral ligament from surrounding inner-ear tissues during separation. This is important because the cochlear lateral wall contains closely associated structures that must remain as intact as possible. A microscopically guided preparation produces tissue more suitable for reliable downstream examination and interpretation.
The preparation centers on exposing the cochlear lateral wall, identifying the spiral ligament under microscopy, and carefully detaching it from surrounding tissues. Researchers then preserve the isolated tissue for histology, immunolabeling, or molecular analysis. The essential procedural goal is controlled separation while minimizing disruption to nearby cochlear structures.
Three supported analytical routes are histology, immunolabeling, and molecular analysis. Histology is suited to examining tissue structure, whereas immunolabeling supports investigation of specific biological features within the preparation. Molecular analysis adds information at the molecular level. Selecting among these approaches depends on whether the study emphasizes architecture, localization, or molecular characteristics.
This preparation is useful when researchers need to examine the cochlear lateral wall in relation to inner-ear development, auditory function, or pathology. It can also support investigations of ion regulation and hearing-associated mechanisms. By providing tissue for multiple analytical methods, the procedure connects microscopic cochlear organization with broader biological questions about hearing.