Tsa-201 Cells
Tsa-201 Cells - Related Videos
09:36
Visualization of Mitochondrial DNA Replication in Individual Cells by EdU Signal Amplification
0 Views •
Cited by 11 •
2010
We developed a sensitive technique to label newly synthesized mitochondrial DNA (mtDNA) in individual cells in order to study mtDNA biogenesis. The technique combines the incorporation of EdU together with a tyramide signal amplification (TSA) protocol to visualize mtDNA replication within subcellular compartments of neurons.
07:14
Assessing Phagocytic Clearance of Cell Death in Experimental Stroke by Ligatable Fluorescent Probes
0 Views •
Cited by 4 •
2014
We present a new fluorescence technique for selective in situ labeling of active phagocytic cells, which clear off cell corpses in stroke. The approach is important for assessing brain reaction to ischemia because only a small proportion of phagocytes present in ischemic brain participate in clearance of cell death.
12:04
Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy
0 Views •
Cited by 39 •
2012
We present a simple agarose overlay platform to grow 3D multicellular spheroids using neuroendocrine cancer cell lines. This method provides a very convenient way to examine the effect of therapeutic drugs on the neuroendocrine tumor cells. It could also help us establish human neuroendocrine tumor spheroids for cancer therapy.
13:36
Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
0 Views •
Cited by 10 •
2010
We demonstrate here that epigenetic reprogramming via Somatic Cell Nuclear Transfer (SCNT) can be used as a tool to generate mouse models with pre-defined T cell receptor (TCR) specificities. These transnuclear mice express the corresponding TCR from their endogenous locus under the control of the endogenous promoter.
07:15
Invasion of Human Cells by a Bacterial Pathogen
0 Views •
Cited by 44 •
2011
A general protocol for the study of invasion of host cells by a bacterial pathogen, focusing on Staphylococcus aureus and human endothelial cells.
FAQs
The SV40 large T antigen supports replication of transfected plasmids that contain an SV40 origin. This can increase the amount of expression template available inside the cells and promote high-level, transient production of the encoded recombinant protein. The mechanism is especially useful when researchers need substantial protein expression for short-term functional or biochemical experiments.
Their expression system is suited to experiments in which protein production is measured after transfection without establishing a permanently modified cell population. Because plasmid replication and expression are transient, researchers can examine the encoded protein during a defined experimental period. This supports rapid testing of constructs and functional analysis while retaining routine cultured-cell handling.
Tsa-201 cells support heterologous expression, meaning a protein can be produced in cells different from its original biological source. This is valuable for ion channels, receptors, and other membrane proteins because expression can be connected directly to cellular function. Researchers can therefore investigate protein behavior using physiological or molecular readouts in a cultured-cell setting.
These approaches examine different consequences of recombinant protein expression. Electrophysiology can assess functional activity associated with ion channels or other electrically relevant proteins, fluorescence imaging can reveal labeled or fluorescent signals in cells, and biochemical assays can evaluate protein-related molecular properties. Using these readouts together helps connect expression with localization, activity, or biochemical behavior.
A typical workflow begins with routine culture of the cells, followed by transfection with a plasmid encoding the recombinant protein. When the plasmid contains an SV40 origin, the expressed SV40 large T antigen can support its replication. After allowing transient expression, researchers assess the protein with electrophysiology, fluorescence imaging, or biochemical assays.
This cell line is particularly useful when an experiment requires cultured-cell production of a recombinant protein together with a functional measurement. It can support studies of ion channels, receptors, and other membrane proteins, as well as broader heterologous expression work. The combination of routine culture, transient high-level expression, and multiple assay options makes it adaptable to biomedical research.