Chicken Primary Bursal Cells
Chicken Primary Bursal Cells - Related Videos
07:26
An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis
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Cited by 14 •
2018
Here we describe the isolation of chicken primary bursal cells from the bursa of Fabricius, the culture and infection of the cells with infectious bursal disease virus, and the quantification of viral replication.
15:42
Xenotransplantation of Human Stem Cells into the Chicken Embryo
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Cited by 31 •
2010
In this paper we present a method for transplanting human stem cells into various regions of the central nervous system of the chicken embryo. This provides an in vivo model for assessing the proliferation and differentiation of various types of human stem cells in embryonic tissue environments.
13:06
In Ovo Electroporations of HH Stage 10 Chicken Embryos
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Cited by 19 •
2007
Chick in ovo electroporation is a technique which allows genetic manipulation of the avian embryo. Common applications of this technique include functional analysis of genes and putative enhancer elements. This video demonstrates neural tube electroporation in HH 10 chick embryos. Injection technique and proper egg handling are discussed.
10:14
In Ovo Electroporation in the Chicken Auditory Brainstem
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Cited by 6 •
2017
Auditory brainstem neurons of avians and mammals are specialized for fast neural encoding, a fundamental process for normal hearing functions. These neurons arise from distinct precursors of embryonic hindbrain. We present techniques utilizing electroporation to express genes in the hindbrain of chicken embryos to study gene function during auditory development.
10:57
Chicken Embryo Spinal Cord Slice Culture Protocol
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Cited by 3 •
2013
Slice cultures facilitate the manipulation of embryo development by gene and pharmacological perturbations. However, culture conditions must ensure that normal development can proceed within the reduced environment of the slice. We illustrate a protocol that facilitates normal spinal cord development to proceed for at least 24 hr.
FAQs
Their value comes from preserving key features of bursal tissue after isolation and during controlled culture. This can make observed changes in viability, gene expression, or cytokine production more informative for physiological immune responses than results obtained only from an immortalized cell line. The model therefore links cellular measurements with avian B-cell development and early immune function.
Researchers can evaluate three complementary response classes: whether cells remain viable, which genes change expression, and how cytokine production shifts after stimulation or infection. Together, these measurements distinguish general cellular damage from regulated immune activity more effectively than relying on a single endpoint. Comparing these readouts across conditions helps characterize early host responses.
Exposure to infection, pathogen-derived signals, or other experimental stimuli can alter cellular behavior without requiring analysis of the whole bursal organ. The resulting changes provide an ex vivo view of how bursal cells react at an early stage. This is especially useful for connecting a defined challenge with measurable immune or disease-related responses.
They offer a physiologically relevant alternative because they originate directly from bursal tissue rather than from an immortalized culture. That distinction matters when investigators want responses linked to avian immune maturation, including changes in viability, gene expression, or cytokine production. Immortalized lines can serve as complementary systems, while primary cells provide context closer to the source tissue.
A typical study begins with fresh isolation from the bursa of Fabricius, followed by maintenance under controlled culture conditions. Investigators then expose the cells to infection, pathogen-derived signals, or another defined stimulus and assess viability, gene expression, or cytokine production. This sequence relates the experimental challenge directly to cellular outcomes in an ex vivo setting.
Controlled culture conditions help create a consistent environment for observing responses to an experimental challenge. This makes comparisons of viability, gene expression, and cytokine production easier to interpret because measured differences can be related to the stimulus under investigation. The source material does not identify one universal culture recipe, so conditions must remain aligned with the specific experiment.
They allow investigators to examine how bursal immune cells respond to vaccine-related experimental stimuli under ex vivo conditions. Measurements such as viability, gene expression, and cytokine production can reveal cellular changes associated with that response. Because the cells are connected to the organ involved in avian B-cell development, the model adds context to studies of immune maturation and vaccination.
In infection research, the model can connect a pathogen or pathogen-derived signal with early changes in bursal-cell behavior. Investigators can examine whether a challenge is associated with altered viability, gene expression, or cytokine production, helping characterize host-pathogen interactions and disease mechanisms. This focus supports analysis of cellular events that may contribute to broader avian immune responses.