Extrahepatic Cholangiography
Extrahepatic Cholangiography - Related Videos
07:54
Isolation of Neonatal Extrahepatic Cholangiocytes
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Cited by 17 •
2014
A technique to isolate cholangiocytes from the extrahepatic bile ducts of neonatal mice is described. The ducts are meticulously dissected, and then cells are isolated by outgrowth in thick collagen gels. This method provides a useful tool for studying extrahepatic bile duct development and pathology.
09:13
Generation of Organoids from Mouse Extrahepatic Bile Ducts
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Cited by 10 •
2019
This protocol describes the production of a mouse extrahepatic bile duct 3-dimensional organoid system. These biliary organoids can be maintained in culture to study cholangiocyte biology. Biliary organoids express markers of both progenitor and biliary cells and are composed of polarized epithelial cells.
06:10
Extrahepatic Bile Duct and Gall Bladder Dissection in Nine-Day-Old Mouse Neonates
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Cited by 3 •
2022
For the observation of murine neonatal bile duct disorders, an intact bile duct and efficient preparation are required. Therefore, a new approach for isolating the entire extrahepatic bile duct system in murine neonates was successfully developed while maintaining the integrity of the bile duct.
04:02
Laparoscopic Cholecystectomy with Indocyanine Green Fluorescence: Choledochoscopic Stone Extraction and Primary Duct Suture
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2025
The integrated technique described in this protocol achieves real-time biliary mapping via indocyanine green (ICG) fluorescence, minimally invasive stone clearance under direct visualization, and secure primary duct repair (6-0 PDS), eliminating T-tube drainage.
07:23
Using a Whole-mount Immunohistochemical Method to Study the Innervation of the Biliary Tract in Suncus murinus
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Cited by 2 •
2017
A whole-mount immunohistochemical approach, to visualize neurofilament protein expression in the extrahepatic biliary tract in Suncus murinus. is presented here. This protocol can be used to analyze the innervation of all visceral organs in S. murinus or other species.
FAQs
Radiopaque contrast fills the biliary tree and creates a visible outline on X-ray images. This makes the caliber and continuity of ducts easier to assess, allowing clinicians to recognize areas where the normal passage is interrupted or altered. The resulting anatomical detail supports evaluation of narrowing, leakage, stones, and obstructing lesions that may not be distinguishable without contrast.
Obstruction can impair the normal flow of bile, creating conditions associated with microbial invasion and inflammatory responses. Extrahepatic Cholangiography helps locate structural problems that may interfere with drainage, such as strictures, stones, or lesions. In immunology and infection research, correlating these findings with cholangitis helps explain how altered biliary anatomy relates to infection-related inflammation.
Different changes in the outlined ducts provide clues to distinct structural problems. A stone or obstructing lesion can interrupt the expected passage, a stricture can produce localized narrowing, and a leak can indicate that contrast has escaped the duct system. Recognizing these patterns helps connect the imaging appearance with impaired bile flow and possible complications.
The biliary tree is reached either during endoscopic retrograde cholangiopancreatography or through percutaneous access. Radiopaque contrast is then introduced into the ducts, and X-ray images are obtained as the contrast outlines the extrahepatic anatomy. These two access routes provide the procedural means for examining duct continuity, narrowing, leakage, stones, and obstructing lesions.
It is especially relevant when researchers need to examine how bile duct abnormalities relate to cholangitis and infection-associated inflammation. Imaging can show whether narrowing, stones, leaks, or obstructing lesions are present while the broader investigation considers microbial invasion and immune responses. This connects structural evidence of impaired drainage with the inflammatory complications observed in biliary disease.
Findings can identify abnormalities that require drainage or another intervention intended to restore bile flow. In clinical and research contexts, improvement in duct passage provides a structural basis for interpreting changes in infection or inflammation. Thus, the examination contributes both anatomical evidence and a way to relate correction of obstruction to complications associated with cholangitis.