A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Title
- 00:58Extrahepatic Bile Duct (EHBD) Isolation
- 02:36Biliary Organoid Culture
- 03:48EHBD Organoid Passage and Storage
- 05:01EHBD Organoid Processing for Paraffin Embedding
- 07:07Results: Representative EHBD Characterization and Analysis
- 08:24Conclusion
This protocol describes the production of a mouse extrahepatic bile duct 3-dimensional organoid system. These biliary organoids can be maintained in culture to study cholangiocyte biology. Biliary organoids express markers of both progenitor and biliary cells and are composed of polarized epithelial cells.
