Immune Cell Analysis
Immune Cell Analysis - Related Videos
12:14
Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
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Cited by 80 •
2016
Inflammation plays a central role in the pathogenesis of ischemic stroke. Increasing evidence suggests that it acts as a double-edged sword which exacerbates early brain injury, but also contributes to later repair. This protocol describes the isolation of immune cells from the ischemic brain and their subsequent flow cytometric phenotyping.
04:38
Analysis of Immune Cells in Mouse Nervous Tissues Using Flow Cytometry
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2025
Source: Hidmark, A. S., et al. Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry. J. Vis. Exp. (2017). This video demonstrates the use of flow cytometry to analyze immune cells in mouse sciatic nerves and dorsal root ganglia (DRGs). The process involves labeling immune cells with fluorophore- and biotin-tagged antibodies, stabilizing and permeabilizing the cells, and using fluorescent markers to detect cell-surface and nuclear...
15:15
Flow Cytometry Analysis of Immune Cells Within Murine Aortas
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Cited by 87 •
2011
This paper presents a flow cytometry-based method to investigate the immune composition of aortas. The paper also illustrates an additional technique that allows examining surrounding adventitia and vessel wall separately. This method opens possibilities to perform phenotypical analyses of aortic leukocytes and apply several immunological assays for atherosclerosis studies.
07:26
Isolation of Functional Cardiac Immune Cells
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Cited by 9 •
2011
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
15:39
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
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Cited by 8 •
2019
Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.
FAQs
Fluorescently labeled antibodies bind selected cell-surface or intracellular markers, allowing researchers to distinguish immune populations and characterize their phenotype. Surface markers reveal features exposed on the cell, whereas intracellular markers provide information from within the cell. This distinction helps analysis move beyond simple cell counts and identify biologically different immune populations.
Phenotypic measurements describe which markers immune cells carry and how abundant particular populations are. Functional assays instead examine what those cells do, such as producing cytokines, proliferating, or displaying cytotoxicity. Combining both perspectives can show whether a population is merely present or is actively responding in a biologically meaningful way.
Innate and adaptive immune populations can respond differently to pathogens, vaccination, or treatment, so separating them provides more informative interpretation than evaluating immune cells as one group. Measuring changes in each population can reveal which arm of immunity is associated with a response, supporting analysis of immune protection and treatment-related effects.
A supported workflow begins by identifying and measuring immune cell populations, then characterizing their markers with fluorescently labeled antibodies when appropriate. Functional assays can follow to assess cytokine production, proliferation, or cytotoxicity. Reviewing abundance, phenotype, and function together produces a more complete assessment than relying on any single measurement type.
In infection research, these measurements help determine how immune populations respond to pathogens and how those responses change after vaccination or treatment. Researchers can examine population abundance, marker-defined phenotype, and functional activity to profile disease-related immune changes. The resulting information may help evaluate immune protection or identify responses associated with intervention.
The resulting measurements can support disease profiling, biomarker discovery, evaluation of immune protection, and development of targeted therapies. Abundance data may describe the scale of an immune response, phenotype data may identify distinguishing cellular features, and functional results may indicate activity such as cytokine production, proliferation, or cytotoxicity.