Splanchnic Nerve Isolation
Splanchnic Nerve Isolation - Related Videos
05:29
Axoplasm Isolation from Rat Sciatic Nerve
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Cited by 6 •
2010
We demonstrate a protocol for axoplasm isolation from adult rat sciatic nerve based on dissection of nerve fascicles and incubation in hypotonic medium to release myelin and lyse non-axonal structures, followed by extraction of the remaining axon-enriched material.
10:32
In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments
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Cited by 8 •
2016
Mesenteric afferent nerves convey information from the gastrointestinal tract towards the brain regarding normal homeostasis as well as pathophysiology. Gastrointestinal afferent nerve activity can be assessed by mounting isolated intestinal segments with attached afferent nerves into an organ bath, isolating the nerve, and assessing basal as well as stimulated activity.
02:50
Toluidine Blue Staining of Peripheral Nerve Sections: A Technique to Stain Myelinated Nerve Fibers For Peripheral Nerve Structure Visualization
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2025
This video demonstrates the staining technique of resin-embedded, osmium tetroxide-treated rat peripheral nerve sections using toluidine blue. The stained nerve sections help visualize the fine structures of peripheral nerves.
12:03
Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
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Cited by 12 •
2014
Developing biotinylatable fusion proteins has many potential applications in various fields of research. Recombinant protein engineering is a straight forward procedure that is cost-effective, providing high yields of custom-designed proteins.
02:00
Vagus Nerve Stimulation via Chronically Implanted Peripheral Nerve Cuff Electrodes in Rats
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2025
Source: Sanchez, C. A., et al. Preparation of Peripheral Nerve Stimulation Electrodes for Chronic Implantation in Rats. J. Vis. Exp. (2020).This video demonstrates vagus nerve stimulation in an awake rat. A rat with an implanted chronic peripheral nerve cuff electrode and trained in a lever press task is connected to a stimulus generator via an implanted head cap. The vagus nerve is stimulated immediately after each successful lever press, resulting in the expansion of the motor map.
FAQs
The dissection must distinguish the target nerve from the sympathetic trunk, nearby vessels, and connective tissue. This anatomical discrimination is essential because these structures may lie in close proximity while serving different functions. Accurate identification allows investigators to expose the nerve specifically, reducing the risk of attributing recorded or stimulated activity to an adjacent structure.
Maintaining continuity keeps the isolated segment connected along its natural pathway between the sympathetic trunk and visceral targets. That preservation supports investigations of signals traveling through the nerve rather than responses from disconnected tissue. It also makes the preparation suitable for electrophysiological recording, stimulation, tracing, or selective manipulation of visceral neural pathways.
The preparation provides access to sympathetic efferent pathways that influence abdominal and pelvic viscera, as well as visceral afferent pathways that convey information toward the central nervous system. Researchers can therefore examine outgoing autonomic control and incoming organ-related signaling within the same anatomical connection, helping relate peripheral activity to broader visceral neural circuits.
The procedure begins with anatomical identification of the relevant nerve and its relationship to the sympathetic trunk and visceral region. Tissue is then separated carefully to expose the nerve while preserving its continuity. Throughout the dissection, the operator distinguishes neural tissue from vessels and connective tissue, producing a preparation appropriate for focused neural investigation.
An isolated preparation can be used for electrophysiological recording to examine neural activity, stimulation to test pathway responses, tracing to follow anatomical connections, or selective manipulation to investigate pathway function. These approaches provide complementary information about signal transmission and connectivity, allowing researchers to study how sympathetic and visceral pathways participate in autonomic regulation.
The technique links anatomical access with functional analysis of autonomic circuits. By focusing on nerves connecting the sympathetic trunk with abdominal and pelvic viscera, researchers can examine how internal organs communicate with the central nervous system and how sympathetic pathways regulate visceral function. This makes the preparation relevant to studies of peripheral signaling and organ-directed neural control.