Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove


We describe a protocol detailing the isolation of murine colonic crypts for the development of 3-dimensional colonoids. The established colonoids can then be terminally differentiated to reflect the cellular composition of the host epithelium prior to receiving an inflammatory challenge or being directed to establish an epithelial monolayer.


Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter