Micro-Drive Implant for Recording Daily Neural Activity in Juvenile Mice

June 02, 2023

A Lightweight Drive Implant for Chronic Tetrode Recordings in Juvenile Mice (Video) | JoVE

01:55 min

•

Micro-Drive Design and Construction (Video) | JoVE

03:25 min

•

Surgical Implantation of the Micro-Drive on the Mouse Skull (Video) | JoVE

00:57 min

•

Recovery after Micro-Drive Implantation (Video) | JoVE

01:00 min

•

Robert J. Pendry², Lilyana D. Quigley², Lenora J. Volk^3,4, Brad E. Pfeiffer³

¹Department of Neuroscience,UT Southwestern Medical Center, ²Neuroscience Graduate Program,UT Southwestern Medical Center, ³O’Donnell Brain Institute,UT Southwestern Medical Center, ⁴Department of Psychiatry,UT Southwestern Medical Center

Summary

Here, we describe a micro-drive design, surgical implantation procedure, and post-surgery recovery strategy that allow for chronic field and single-unit recordings from multiple brain regions simultaneously in juvenile and adolescent mice across a critical developmental window from postnatal day 20 (p20) to postnatal day 60 (p60) and beyond.