Mouse Retina OCT Fibergram Alignment with Confocal Images

June 30, 2023

Advancements in In Vivo and Ex Vivo Retinal Imaging for Improved Glaucoma Diagnosis and Treatment (Video) | JoVE

01:26 min

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In Vivo Visible Light Optical Coherence Tomography (OCT) Mouse Retinal Imaging (Video) | JoVE

02:20 min

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Ex Vivo Confocal Imaging of Mouse Retina (Video) | JoVE

02:34 min

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Alignment of In Vivo and Ex Vivo Mouse Retinal Images (Video) | JoVE

01:25 min

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Shichu Chang, Wenjin Xu, Weijia Fan, John A. McDaniel, Marta Grannonico, David A. Miller, Mingna Liu, Hao F. Zhang, Xiaorong Liu^3,4,5

¹Department of Biology,University of Virginia, ²Department of Biomedical Engineering,Northwestern University, ³Department of Ophthalmology,University of Virginia, ⁴Program in Fundamental Neuroscience,University of Virginia, ⁵Department of Psychology,University of Virginia

Summary

The present protocol outlines the steps for aligning in vivo visible-light optical coherence tomography fibergraphy (vis-OCTF) images with ex vivo confocal images of the same mouse retina for the purpose of verifying the observed retinal ganglion cell axon bundle morphology in the in vivo images.