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Research
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Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures (Video) | JoVE
Preparation of Photo-Oxidized Outer Segment Tips and Fragments (Video) | JoVE
Characterization of the Lipofuscin-Like Granule Spectrum and Composition (Video) | JoVE
Assessing the Effects of Lipofuscin-Like Granules on RPE Phagocytosis: Total Consumptive Capacity (Video) | JoVE
该协议描述了高度分化和极化的人视网膜色素上皮(RPE)培养物中的脂褐素积累模型和改进的外段(OS)吞噬测定,以检测RPE的总OS消耗/降解能力。这些方法克服了以前的脂褐素模型和经典的脉搏追逐外段吞噬测定的局限性。
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