Stria Vascularis Microdissection for Single-Nucleus Sequencing or Immunostaining

April 21, 2023

Dissection of Adult Mouse Stria Vascularis for Single-Nucleus Sequencing or Immunostaining (Video) | JoVE

01:31 min

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Mouse Inner Ear Extraction and Stria Vascularis Dissection (Video) | JoVE

03:49 min

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Preparation of Stria Vascularis Single-Nucleus Suspension (Video) | JoVE

01:28 min

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Immunostaining of Mouse Stria Vascularis and Tissue Mounting (Video) | JoVE

02:46 min

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Dillon Strepay, Rafal Olszewski, Ian Taukulis, J. Dixon Johns, Shoujun Gu, Michael Hoa

¹Auditory Development and Restoration Program, National Institute on Deafness and Other Communication Disorders,National Institutes of Health

Summary

The stria vascularis is vital to the generation of endocochlear potential. Here, we present the dissection of the adult mouse stria vascularis for single-nucleus sequencing or immunostaining.