Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator (Video) | JoVE

Kaede Saito; Yuta Hiraike; Misato Oguchi; Toshimasa Yamauchi

doi:10.3791/65265

Semi-Automated Isolation of Murine White Adipose Tissue

May 19th, 2023

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator (Video) | JoVE

02:39 min

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Isolation, Digestion, Filtration and Cell Plating of Murine Adipose Tissue (Video) | JoVE

03:47 min

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Kaede Saito^1,2, Yuta Hiraike^1,2,3, Misato Oguchi², Toshimasa Yamauchi²

¹Division for Health Service Promotion, The University of Tokyo, ²Department of Diabetes and Metabolic Diseases, Graduate School of Medicine, The University of Tokyo, ³The University of Tokyo Excellent Young Researcher Program, The University of Tokyo

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This protocol describes the semi-automated isolation of the stromal vascular fraction (SVF) from murine adipose tissue to obtain preadipocytes and achieve adipocyte differentiation in vitro. Using a tissue dissociator for collagenase digestion reduces experimental variation and increases reproducibility.

Article

Semi-Automated Isolation of Murine White Adipose Tissue

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