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Optimization of Performance Parameters of the TAGGG Telomere Length Assay (Video) | JoVE
A2780 Cells Genomic DNA Isolation and Analysis (Video) | JoVE
Southern Blotting, Hybridization, and Chemiluminescence Detection of Genomic DNA Isolated from A2780 Cells (Video) | JoVE
ここでは、非放射性化学発光検出を用いてテロメア長を定量するためのプロトコルについて、TAGGGテロメア長アッセイキットの様々な性能パラメータ(緩衝液量やプローブ濃度など)の最適化を中心に詳細に説明する。
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