Porphyrin-Modified Beads in Flow Cytometry

March 24, 2023

Porphyrin-Modified Beads for Use as Compensation Controls in Flow Cytometry (Video) | JoVE

01:03 min

•

Preparation of Meso-Tetra(4-Carboxyphenyl) Porphine (TCPP) Stock Solution (Video) | JoVE

01:33 min

•

Combining Amine-Functionalized Polystyrene Beads with TCPP Solution (Video) | JoVE

01:00 min

•

Labeling Amine-Functionalized Polystyrene Beads with TCPP (Video) | JoVE

02:13 min

•

Quality Check (QC) of the TCPP Beads by Flow Cytometry (Video) | JoVE

01:58 min

•

TCPP Bead Filtration (Video) | JoVE

02:56 min

•

William Bauta, Marcia Grayson, Rossella Titone, Jennifer Rebeles, Vivienne I. Rebel

¹bioAffinity Technologies

Summary

The protocol describes how porphyrin-based compensation beads for flow cytometry are prepared by the reaction of amine-functionalized polystyrene beads with the porphyrin TCPP and the amide coupling reagent EDC. A filtration procedure is used to reduce the particulate byproducts.