Real-Time Measurements of Calcium and Contractility Parameters in hiPSC-CMs

May 26, 2023

Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (Video) | JoVE

01:21 min

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Contractility Measurements in hiPSC-CMs (Video) | JoVE

02:30 min

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Calcium Transient Measurements in hiPSC-CMs (Video) | JoVE

01:29 min

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Contractility and Calcium Transient Measurements Data Analysis (Video) | JoVE

01:40 min

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Rafeeh Dinani², Emmy Manders^2,3, Michiel Helmes^2,3, Lili Wang, Bjorn C. Knollmann, Diederik W. D. Kuster², Jolanda van der Velden²

¹Division of Physiology, Amsterdam UMC,Vrije University, ²Heart Failure & Arrhythmias,Amsterdam Cardiovascular Sciences, ³CytoCypher BV, ⁴Division of Clinical Pharmacology,Vanderbilt School of Medicine

Summary

Here, an established method is described to perform contractility and calcium measurements in human induced pluripotent stem cell-derived cardiomyocytes using an optics-based platform. This platform enables researchers to study the effect of mutations and the response to various stimuli in a rapid and reproducible manner.