Neutrophil Functional Assays for Screening Different Signaling Pathways

February 09, 2024

Balancing Speed, Cost, and Accuracy in Neutrophil Function Assessment with NeutroFun Screen Protocol (Video) | JoVE

02:02 min

•

Neutrophil Isolation and Activation

02:54 min

•

Nitrotetrazolium Blue Chloride (NBT) Assay for Evaluating ROS Production (Video) | JoVE

02:59 min

•

Neutrophil Phagocytosis Assay

01:58 min

•

Real-Time Polymorphonuclear Neutrophils (PMN) Chemotaxis Assay (Video) | JoVE

01:26 min

•

Neutrophil Extracellular Trap (NET) Suggestive Assay (Video) | JoVE

01:22 min

•

Isabelle Souza Luz, Raquel Takaya, Daiane Gonzaga Ribeiro, Natanael Sales Silva, Lucas Fontes, Mariana S. Castro, Wagner Fontes

¹Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology,University of Brasilia, ²Institute of Physics,University of Brasilia

Summary

This protocol features a set of neutrophil functional assays to be used as a screening method to cover functions from different signaling pathways. The protocol includes an initial and simple evaluation of cell viability, purity, reactive oxygen species production, real-time migration, phagocytosis, and a preliminary suggestion of neutrophil extracellular traps.