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Closed System Production of Adherent Cells Based on Dissolvable Microcarriers

July 07, 2023
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Advancing Cell Therapy Manufacturing with Dissolvable Microcarriers (Video) | JoVE

02:13 min
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Preparation of the Stirred-Tank Bioreactor for Large-Scale Production of Human Mesenchymal Stem Cells

01:52 min
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Two-Tiered Expansion of Human Mesenchymal Stem Cells from 5-Liter to 15-Liter Bioreactors and Automated Cell Formulation from a 15-Liter Bioreactor (Video) | JoVE

06:10 min
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1Department of Biomedical Engineering, School of Medicine,Tsinghua University, 2Beijing CytoNiche Biotechnology Co. Ltd.

Summary

Automatic Translation

Here, we present a protocol to achieve the large-scale manufacturing of adherent cells through a fully closed system based on GMP-grade dissolvable microcarriers. The cultivation of human mesenchymal stem cells, HEK293T cells, and Vero cells was validated and met both quantity demands and quality criteria for the cell and gene therapy industry.

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