Image Analysis of Immunohistochemically Stained Endometrial Tissue

October 13, 2023

Advancing Reproductive Immunology with a Protocol for the Quantitative Evaluation of Endometrial Immune Cells (Video) | JoVE

01:12 min

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Dehydration of Human Endometrial Tissue for Sectioning

00:54 min

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Human Endometrial Tissue Embedding and Sectioning for Immunohistochemistry and Digital Image Analysis (Video) | JoVE

01:59 min

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Tissue Immunohistochemical Staining of Sectioned Human Endometrial Tissue

01:17 min

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Tissue Scanning and Image Analysis of Immunohistochemically Stained Human Endometrium (Video) | JoVE

03:10 min

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Cong Chen, Chunyu Huang², Yaya Wu, Zhihong Li, Shuyi Yu, Xiaoyan Chen, Ruochun Lian, Rong Lin, Lianghui Diao², Yong Zeng², Yuye Li²

¹Shenzhen Key Laboratory of Reproductive Immunology for Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center,Shenzhen Zhongshan Urology Hospital, ²Shenzhen Jinxin Medical Technology Innovation Center, Co., Ltd.

Summary

Here, a digital immunohistochemistry image analysis platform was developed and validated to quantitatively analyze the endometrial immune cells of patients with recurrent miscarriages in the window of implantation.