Materials
Name | Company | Catalog Number | Comments |
5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic-6,8,9,11,12,14,15-d7 acid | Cayman Chemical | 334250 | [2H7]-5-ox–TE Internal Standard |
5S-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic-5,6,8,9,11,12,14,15-d8 acid | Cayman Chemical | 334230 | [2H8]-5(S)-HETE Internal Standard |
12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic-5,6,8,9,11,12,14,15-d8 acid | Cayman Chemical | 334570 | [2H8]-12(S)-HETE Internal Standard |
15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic-5,6,8,9,11,12,14,15-d8 acid | Cayman Chemical | 334720 | [2H8]-15(S)-HETE Internal Standard |
20-hydroxy-5Z,8Z,11Z,14Z-eicosatetraenoic-16,16,17,17,18,18-d6 acid | Cayman Chemical | 390030 | [2H6]-20-HETE Internal Standard |
9S-hydroxy-10E,12Z-octadecadienoic-9,10,12,13-d4 acid | Cayman Chemical | 338410 | [2H4]-9(S)-HODE Internal Standard |
13S-hydroxy-9Z,11E-octadecadienoic-9,10,12,13-d4 acid | Cayman Chemical | 338610 | [2H4]-13(S)-HODE Internal Standard |
9α,11α,15S-trihydroxy-prosta-5Z,13E-dien-1-oic-17,17,18,18,19,19,20,20,20-d4 acid | Cayman Chemical | 316010 | [2H4]-PGF2α Internal Standard |
9α,11α,15S-trihydroxy-(8β)-prosta-5Z,13E-dien-1-oic-3,3,4,4-d4 acid | Cayman Chemical | 316350 | [2H4]-8-iso-PGF2a Internal Standard |
9α,11β.,15S-trihydroxy-prosta-5Z,13E-dien-1-oic-3,3,4,4-d4 acid | Cayman Chemical | 10008989 | [2H4]-11β-PGF2 Internal Standard |
9α,15S-dihydroxy-11-oxo-prosta-5Z,13E-dien-1-oic-17,17,18,18,19,19,20,20,20-d4 acid | Cayman Chemical | 312010 | [2H4]-PGD2 Internal Standard |
9-oxo-11α,15S-dihydroxy-prosta-5Z,13E-dien-1-oic-17,17,18,18,19,19,20,20,20-d4 acid | Cayman Chemical | 314010 | [2H4]-PGE2 Internal Standard |
5S,12R-dihydroxy-6Z,8E,10E,14Z-eicosatetraenoic-6,7,14,15-d4 acid | Cayman Chemical | 320110 | [2H4]-LTB4 Internal Standard |
9α,11,15S-trihydroxy-thromba-5Z,13E-dien-1-oic-3,3,4,4-d4 acid | Cayman Chemical | 319030 | [2H4]-TxB2 Internal Standard |
5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid | Cayman Chemical | 34250 | 5-ox–TE Standard |
12-oxo-5Z,8Z,10E,14Z-eicosatetraenoic acid | Cayman Chemical | 34580 | 12-ox–TE Standard |
15-oxo-5Z,8Z,11Z,13E-eicosatetraenoic acid | Cayman Chemical | 34730 | 15-ox–TE Standard |
5R-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid | Cayman Chemical | 34225 | 5(R)-HETE Standard |
5S-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid | Cayman Chemical | 34230 | 5(S)-HETE Standard |
8R-hydroxy-5Z,9E,11Z,14Z-eicosatetraenoic acid | Cayman Chemical | 34350 | 8(R)-HETE Standard |
8S-hydroxy-5Z,9E,11Z,14Z-eicosatetraenoic acid | Cayman Chemical | 34360 | 8(S)-HETE Standard |
11R-hydroxy-5Z,8Z,12E,14Z-eicosatetraenoic acid | Cayman Chemical | 34505 | 11(R)-HETE Standard |
11S-hydroxy-5Z,8Z,12E,14Z-eicosatetraenoic acid | Cayman Chemical | 34510 | 11(S)-HETE Standard |
12R-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid | Cayman Chemical | 34560 | 12(R)-HETE Standard |
12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid | Cayman Chemical | 34570 | 12(S)-HETE Standard |
15R-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid | Cayman Chemical | 34710 | 15(R)-HETE Standard |
15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid | Cayman Chemical | 34720 | 15(S)-HETE Standard |
20-hydroxy-5Z,8Z,11Z,14Z-eicosatetraenoic acid | Cayman Chemical | 90030 | 20-HETE Standard |
9R-hydroxy-10E,12Z-octadecadienoic acid | Cayman Chemical | 38405 | 9(R)-HODE Standard |
9S-hydroxy-10E,12Z-octadecadienoic acid | Cayman Chemical | 38410 | 9(S)-HODE Standard |
13R-hydroxy-9Z,11E-octadecadienoic acid | Cayman Chemical | 38605 | 13(R)-HODE Standard |
13S-hydroxy-9Z,11E-octadecadienoic acid | Cayman Chemical | 38610 | 13(S)-HODE Standard |
9α,11α,15S-trihydroxy-prosta-5Z,13E-dien-1-oic acid | Cayman Chemical | 16010 | PGF2α Standard |
9α,11α,15S-trihydroxy-(8β)-prosta-5Z,13E-dien-1-oic acid | Cayman Chemical | 16350 | 8-iso-PGF2α Standard |
9α,11β,15S-trihydroxy-prosta-5Z,13E-dien-1-oic acid | Cayman Chemical | 16520 | 11β-PGF2 Standard |
9α,15S-dihydroxy-11-oxo-prosta-5Z,13E-dien-1-oic acid | Cayman Chemical | 12010 | PGD2 Standard |
9-oxo-11α,15S-dihydroxy-(8β)-prosta-5Z,13E-dien-1-oic acid | Cayman Chemical | 14350 | 8-iso-PGE2 Standard |
9-oxo-11α,15S-dihydroxy-prosta-5Z,13E-dien-1-oic acid | Cayman Chemical | 14010 | PGE2 Standard |
5S,12R-dihydroxy-6Z,8E,10E,14Z-eicosatetraenoic acid | Cayman Chemical | 20110 | LTB4 Standard |
9α,11,15S-trihydroxythromba-5Z,13E-dien-1-oic acid | Cayman Chemical | 19030 | TxB2 Standard |
Phosphate Buffered Saline | GIBCO, by Life Technologies | 14190 | |
Diethyl Ether | Sigma-Aldrich | 346136 | |
Dichloromethane | Acros Organics | 61030-1000 | anhydrous |
N,N-diisopropylethyl amine | Sigma-Aldrich | 387649 | |
Pentafluorylbenzyl bromide | Sigma-Aldrich | 101052 | |
Hydrochloric Acid | Sigma-Aldrich | 320331 | |
Potassium Hydroxide | Fluka | 00650 | |
Acetonitrile | Fisher Scientific | A996-4 | |
Methanol | Fisher Scientific | A454-4 | |
Chloroform | Fisher Scientific | 366927 | |
Hexane | Fisher Scientific | H303-4 | |
Isopropanol | Fisher Scientific | A464-4 | |
Ethanol | Decon Laboratories | 2716 | |
Water | Fisher Scientific | W7-4 | |
Pasteur Pipets | Fisher Scientific | 13-678-200 | |
10 mL Glass Centrifuge Tubes | Kimble Chase | 73785-10 | Screw cap |
Phenolic Screw Caps | Kimble Chase | 73802-13415 | |
Chiralcel ADH Column | Chiral Technologies | 19325 | |
HPLC vials | Waters | 60000751CV | |
HPLC inserts | Waters | WAT094171 |
References
- Needleman, P., Turk, J., Jakschik, B. A., Morrison, A. R., Lefkowith, J. B.
Arachidonic acid metabolism. Annu. Rev. Biochem. 55, 69-102 (1986). - Nikolaev, V., Reddanna, P., Whelan, J., Hildenbrandt, G., Reddy, C. C. Stereochemical nature of the products of linoleic acid oxidation catalyzed by lipoxygenases from potato and soybean. Biochem. Biophys. Res. Commun. 170, 491-496 (1990).
- Panigrahy, D., Kaipainen, A., Greene, E. R., Huang, S. Cytochrome P450-derived eicosanoids: the neglected pathway in cancer. Cancer. Metastasis. Rev. 29, 723-735 (2010).
- Basu, S. Bioactive eicosanoids: role of prostaglandin F(2alpha) and F-isoprostanes in inflammation and oxidative stress related pathology. Mol. Cells. 30, 383-391 (2010).
- Sanchez-Mejia, R. O., Mucke, L. Phospholipase A2 and arachidonic acid in Alzheimer's disease. Biochim. Biophys. Acta. 1801, 784-790 (2010).
- Hersberger, M. Potential role of the lipoxygenase derived lipid mediators in atherosclerosis: leukotrienes, lipoxins and resolvins. Clin. Chem. Lab Med. 48, 1063-1073 (2010).
- Allayee, H., Roth, N., Hodis, H. N. Polyunsaturated fatty acids and cardiovascular disease: implications for nutrigenetics. J. Nutrigenet. Nutrigenomics. 2, 140-148 (2009).
- Capdevila, J. H., Falck, J. R. Biochemical and molecular properties of the cytochrome P450 arachidonic acid monooxygenases. Prostaglandins Other Lipid Mediat. , 68-69 (2002).
- Ciccimaro, E., Blair, I. A. Stable-isotope dilution LC-MS for quantitative biomarker analysis. Bioanalysis. 2, 311-341 (2010).
- Blair, I. A. Electron-capture negative-ion chemical ionization mass-spectrometry of lipid mediators. Methods in Enzymology. 187, 13-23 (1990).
- Singh, G., Gutierrez, A., Xu, K., Blair, I. A. Liquid chromatography/electron capture atmospheric pressure chemical ionization/mass spectrometry: analysis of pentafluorobenzyl derivatives of biomolecules and drugs in the attomole range. Analytical Chemistry. 72, 3007-3013 (2000).
- Blair, I. A. Encyclopedia of Mass Spectrometry. Capriol, R. M., Gross, M. L. , Elsevier. 283-307 (2005).
- Lee, S. H., Blair, I. A. Targeted chiral lipidomics analysis by liquid chromatography electron capture atmospheric pressure chemical ionization mass spectrometry (LC-ECAPCI/MS). Methods in Enzymology. 433, 159-174 (2007).