Breast Tissue Assessment

Breast tissue assessment is the systematic evaluation of breast structure and composition to identify normal findings, benign changes, or signs that require further investigation. In clinical practice, providers combine patient history and physical examination with imaging methods such as mammography, ultrasound, or magnetic resonance imaging; when imaging reveals a suspicious area, tissue sampling and histopathology can determine whether abnormal cells are present. This assessment supports early detection, diagnostic classification, and treatment planning for breast cancer and other breast conditions. Accurate interpretation also helps guide follow-up decisions while reducing unnecessary procedures for noncancerous findings.

Breast Tissue Assessment - Related Videos

Research

JoVE Journal - Medicine

Mammosphere Formation Assay from Human Breast Cancer Tissues and Cell Lines

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Cited by 116 •

2015

Floating mammosphere assays can investigate the subset of stem-like breast cancer cells that survive in suspension conditions and show enhanced tumorigenesis when implanted into mice. This protocol provides a convenient in vitro measure of sphere-forming ability, a proxy for in vivo tumorigenesis, while facilitating analysis of the stem-associated transcriptional landscape.

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

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Cited by 4 •

2016

Here we present a protocol to detect miRNA expression in breast cancer patient samples using miRNA in situ hybridization.

Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System

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Cited by 5 •

2021

This protocol describes the construction of an in vitro microphysiological system for studying breast cancer using primary human breast tissue with off the shelf materials.

Research

JoVE Journal - Medicine
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Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

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Cited by 27 •

2014

Activation of latent mutations with adenovirus-Cre into the mammary ductal system results in a clinically relevant metastatic breast cancer. Incorporation of a YFP promoter allows tracking of distal metastatic tumor cells. This model is useful to study latent metastasis, anti-tumor immunity, and for designing novel immunotherapies to treat breast cancer.

Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche

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Cited by 20 •

2015

Protocols are described for studying breast cancer cell migration, proliferation and colonization in a human bone tissue explant model system.

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