/

/

Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche

JoVE Journal
Medicine

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Medicine

Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche

Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche

DOI:

10.3791/52656-v

•

11:41 min

•

March 15, 2015

•

Zachary S. Templeton, Michael H. Bachmann, Rajiv V. Alluri, William J. Maloney, Christopher H. Contag, Bonnie L. King

¹Department of Pediatrics,Stanford University School of Medicine, ²Department of Orthopaedic Surgery,Stanford University School of Medicine

Chapters

00:05Title
02:00Isolating Tissue Fragments from Femoral Heads
02:40Co-culturing to Study Cell Proliferation
04:19Co-culturing to Study Colonization and Cell Number
06:45Measuring Cell Migration in the Co-culture: Towards Bone Tissue Supernatant
09:30Results: Breast Cancer Cell Proliferation, Colonization, and Migration in Co-culture
11:10Conclusion

Summary

Automatic Translation

Protocols are described for studying breast cancer cell migration, proliferation and colonization in a human bone tissue explant model system.

Tags

Bone Metastasis Breast Cancer In Vitro Model Microenvironment Metastatic Niche Cancer Cell Colonization Bioluminescence Imaging Fluorescence Microscopy Flow Cytometry

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Method for Novel Anti-Cancer Drug Development using Tumor Explants of Surgical Specimens

An In Vitro System to Study Tumor Dormancy and the Switch to Metastatic Growth

Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy

The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Three Dimensional Cultures: A Tool To Study Normal Acinar Architecture vs. Malignant Transformation Of Breast Cells

Orthotopic Injection of Breast Cancer Cells into the Mammary Fat Pad of Mice to Study Tumor Growth.

An Ex vivo Model to Study Hormone Action in the Human Breast

Mammosphere Formation Assay from Human Breast Cancer Tissues and Cell Lines

Read Article