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JoVE Journal Biology

Harvesting and Digesting Mouse Skeletal Muscle for 2D Culture of Niche and Stem Cells (Video) | JoVE

Bacterial Inoculation and Paraformaldehyde Inactivation for Caenorhabditis elegans Research (Video) | JoVE

Journal (Biology)

Bacterial Inoculation and Paraformaldehyde Inactivation for Caenorhabditis elegans Research (Video) | JoVE

ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging

Journal (Biology)

ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging

Harvesting and Digesting Mouse Skeletal Muscle for 2D Culture of Niche and Stem Cells (Video) | JoVE

Harvesting Mouse Skeletal Muscle to Culture Stem Cells and Their Niche

Article DOI: 10.3791/200241-v • 04:46 min • June 2nd, 2023

June 2nd, 2023 •

Louai Zaidan*¹, Perla Geara*¹, Matthew J. Borok*¹, Léo Machado¹, Despoina Mademtzoglou¹, Philippos Mourikis¹, Frederic Relaix^1,2,3,4

¹Univ Paris Est Creteil, INSERM, IMRB, F-94010 Creteil, France, ²Ecole nationale vétérinaire d'Alfort, IMRB, F-94700 Maisons-Alfort, France, ³EFS, IMRB, F-94010 Creteil, France, ⁴AP-HP, Hopital Mondor, Service d'histologie, F-94010 Creteil, France

* These authors contributed equally

Tags

Unfractionated Bulk Culture Mouse Skeletal Muscle Niche Stem Cell Quiescence Muscle Functionality Muscle Recovery Cell Types Molecular Signals Myofibers Muscle Stem Cells Physiological Microenvironment Ex Vivo Study Quiescent State Protocol 2D Culture Immunostaining Pax7-positive Cells Cell Amplification Generation Of Quiescent-like Stem Cells Fundamental Questions Translational Questions

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