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Immunology and Infection

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JoVE Journal Immunology and Infection

Isolation and Cryopreservation of Human Peripheral Blood Mononuclear Cells for Bioenergetic Studies (Video) | JoVE

Mouse Model of Oleic Acid-Induced Acute Respiratory Distress Syndrome

Journal (Immunology and Infection)

Mouse Model of Oleic Acid-Induced Acute Respiratory Distress Syndrome

Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions

Journal (Immunology and Infection)

Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions

Isolation and Cryopreservation of Human Peripheral Blood Mononuclear Cells for Bioenergetic Studies (Video) | JoVE

PBMC Collection and Cryostorage for Mitochondrial Bioenergetics Analysis

Article DOI: 10.3791/200888-v • 02:46 min • October 20th, 2023

October 20th, 2023 •

Fabian Dieter¹, Jendrik Grube¹, Tim Birkenhauer¹, Alice Quentin¹, Gunter P. Eckert¹

¹Biomedical Research Center, Institute of Nutritional Sciences, Justus-Liebig-University of Giessen

Tags

Cryopreservation Bioenergetic Evaluation Peripheral Blood Mononuclear Cells Mitochondria Mitochondrial Dysfunction Metabolic Diseases Aging Oxidative Phosphorylation Energetic Homeostasis PBMCs Mitochondrial Function Disease Conditions Human Samples Limitations Cryopreserved Samples Freshly Prepared Cells Isolating PBMCs Cryopreserving PBMCs Bioenergetic Function Cell Number And Viability Adenosine Triphosphate Levels Respiratory Chain Activity Human Blood Samples Clinical Studies Multicentral

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