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Chapters
- 00:05Title
- 01:11Genetic Crossing
- 02:52Isolation of Endosperm Tissues in Arabidopsis
- 03:46Bisulfite Treatment
- 06:20PCR Amplification
- 07:47Sequence Analysis and Determination of DNA Methylation Status: Representative Results
- 09:00Conclusion
Imprinting is a phenomenon in plant and mammal reproduction. DNA methylation plays an important role in mechanisms of imprinting. Isolating endosperm and determining methylation status of imprinted genes in Arabidopsis can be difficult. In this protocol, we describe how to isolate endosperm and determine methylation by bisulfite sequencing.
Tags
DNA MethylationImprinted GenesArabidopsis EndospermEpigenetic MechanismsDNA MethyltransferasesLoss Of DNA MethylationImprintingMaternal And Paternal AllelesReproduction DevelopmentFlowering PlantsDouble Fertilization EventMEDEA GeneFWA GeneDNA Methylation And DemethylationImprinting StatusMethylation PatternIsolate EndospermGenetic Cross
