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Diagnostic Necropsy and Selected Tissue and Sample Collection in Rats and Mice
JoVE 杂志
生物学
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JoVE 杂志 生物学
Diagnostic Necropsy and Selected Tissue and Sample Collection in Rats and Mice

Diagnostic Necropsy and Selected Tissue and Sample Collection in Rats and Mice

DOI:
10.3791/2966-v

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09:13 min

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August 07, 2011

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, , , , ,
1Research Animal Diagnostic Services,Charles River, 2Research Models and Services,Charles River, 3Department of Comparative Medicine,University of Washington

Chapters

  • 00:05Title
  • 00:49Examinations and Postmortem Collection of the Heart, Lung, Liver, Kidney and Spleen
  • 04:01Postmortem Collection of Respiratory Aspirate
  • 05:43Postmortem Collection and Perfusion of the Lungs
  • 06:42Postmortem Collection of the Brain and the Mesenteric Lymph Nodes
  • 08:13Representative Organ Collection
  • 08:41Conclusion

Summary

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This article describes the procedures for conducting a basic postmortem examination of a mouse or rat, and the collection of basic organs, as well as more challenging sample types from for histological, microbiological, and PCR evaluation.

Tags

Diagnostic NecropsySample CollectionRatsMiceHistological ProcessingGross ExaminationHeartLiverLungsKidneysSpleenLung CollectionPerfusionFixativeHistologic EvaluationBrain CollectionMesenteric Lymph NodeInfectious Agents

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