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Preparation of Intact Bovine Tail Intervertebral Discs for Organ Culture
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生物工程
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JoVE 杂志 生物工程
Preparation of Intact Bovine Tail Intervertebral Discs for Organ Culture

Preparation of Intact Bovine Tail Intervertebral Discs for Organ Culture

DOI:
10.3791/3490-v

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13:37 min

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February 02, 2012

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,
1ARTORG Center for Biomedical Engineering,University of Bern

Chapters

  • 00:05Title
  • 02:51Preparing the Bovine Tail for Dissection
  • 04:01Dissection of Intact Bovine Tail Intervertebral Discs
  • 07:02Free-swelling Organ Culture Systems in Beakers / Containers
  • 07:39Organ Culture in Custom-made Specimen Chambers using Glass/PE Containers
  • 08:26Diffusion Experiment to Determine Successful Intervertebral Disc Preparation
  • 09:39Representative Images from Free Swelling and Live/Dead Experiments Performed on IVDs
  • 12:45Conclusion

Summary

自动翻译

自动翻译

This protocol illustrates a harvesting technique for coccygeal bovine intervertebral discs for organ culture for in vitro organ culture.

Tags

Intact Bovine Tail Intervertebral DiscsOrgan CultureIntervertebral Disc (IVD)SpineNucleus Pulposus (NP)Annulus Fibrosus (AF)Cartilaginous EndplatesDegenerative Intervertebral Disc Disease (DDD)Disc HerniationsDiscogenic PainIn Vitro Organ CultureLive Disc CellsMechano-biological AspectsBovine Coccygeal IVDsHuman Lumbar IVDsCell DensityCell PopulationDimensionsBovine Caudal IVD Harvesting TechniquesCartilaginous EndplatesBony EndplatesAvascular OrgansDiffusionCapillary Buds

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