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Biology

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Polymerase Chain Reaction
 

Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies

Article DOI: 10.3791/3998-v 09:00 min May 22nd, 2012
May 22nd, 2012

章节

总结概括

PCR has emerged as a common technique in many molecular biology laboratories. Provided here is a quick guide to several conventional PCR protocols. Because each reaction is a unique experiment, optimal conditions required to generate a product vary. Understanding the variables in a reaction will greatly enhance troubleshooting efficiency, thereby increasing the chance to obtain the desired result.

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Polymerase Chain Reaction PCR Basic Protocol Troubleshooting Optimization Strategies Biological Sciences Technological Advances Microbiology Anton Van Leeuwenhoek Microscope Prokaryotes Keppe And Coworkers Kary Mullis Cetus Corporation Thermal Stable DNA Polymerase Taq DNA Polymerase Amplification Technique Amplicon DNA Template Target Sequence Spurious Results Agarose Gels
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