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Journal / Bioengineering / Lignin nedregulering af
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JoVE Journal Bioengineering
Lignin nedregulering af
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Lignin nedregulering af

Article DOI: 10.3791/51340-v • 14:43 min • July 23rd, 2014
July 23rd, 2014 •
1, 2, 3, 4
1The School of Plant Sciences, University of Arizona, 2Department of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center, Michigan State University, 3The Institute for Sustainable and Renewable Resources, The Institute for Advanced Learning and Research, 4Department of Plant, Soil and Microbial Sciences, Michigan State University

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En dobbeltstrenget RNA-interferens (dsRNAi) teknik anvendes til at nedregulere majs cinnamoyl coenzym A reduktase (ZmCCR1) genet til lavere plante lignin-indhold. Lignin nedregulering fra cellevæggen visualiseres ved mikroskopiske analyser og kvantificeres ved Klason metoden. Sammensætningsmæssige ændringer i hemicellulose og krystallinsk cellulose analyseres.

Tags

Bioteknik , Cinnamoyl-CoA reduktase (CCR) dsRNAi Klason lignin måling cellevæg kulhydrat analyse gaskromatografi (GC)
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