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JoVE Journal Biology

指南现代荧光定量免疫印迹与故障排除策略

Fluorescence Imaging with One-nanometer Accuracy (FIONA)

Journal (Biology)

Fluorescence Imaging with One-nanometer Accuracy (FIONA)

A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy

Journal (Biology)

A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy

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指南现代荧光定量免疫印迹与故障排除策略

Article DOI: 10.3791/52099-v • 11:01 min • November 20th, 2014

November 20th, 2014 •

Samantha L. Eaton¹, Maica Llavero Hurtado¹, Karla J. Oldknow², Laura C. Graham¹, Thomas W. Marchant¹, Thomas H. Gillingwater^3,4, Colin Farquharson², Thomas M. Wishart^1,4

¹Division of Neurobiology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, ²Division of Developmental Biology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, ³Centre for Integrative Physiology, University of Edinburgh, ⁴Euan MacDonald Centre for Motor Neurone Disease Research, University of Edinburgh

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基本的协议，第93，免疫印迹，荧光，LI-COR，蛋白质，定量分析，加载控制

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