Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

Jennifer M. Vojtech; Juliana Cano-Mejia; Matthieu F. Dumont; Raymond W. Sze; Rohan Fernandes

doi:10.3791/52621

Journal

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生物工程

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Biofunctionalized普鲁士蓝纳米粒子多式联运分子成像应用

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

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生物工程

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JoVE 杂志生物工程

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

Biofunctionalized普鲁士蓝纳米粒子多式联运分子成像应用

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10,377 Views

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11:28 min

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April 28, 2015

DOI:

10.3791/52621-v

DOI:

10.3791/52621-v

•

11:28 min

April 28, 2015

•

10343 Views

Jennifer M. Vojtech^1,2, Juliana Cano-Mejia^1,2, Matthieu F. Dumont¹, Raymond W. Sze^1,3, Rohan Fernandes^1,3,4

¹The Sheikh Zayed Institute for Pediatric Surgical Innovation,Children’s National Medical Center ²Fischell Department of Bioengineering,University of Maryland ³Department of Radiology,George Washington University ⁴Department of Pediatrics,George Washington University

Summary

This protocol describes the synthesis of biofunctionalized Prussian blue nanoparticles and their use as multimodal, molecular imaging agents. The nanoparticles have a core-shell design where gadolinium or manganese ions within the nanoparticle core generate MRI contrast. The biofunctional shell contains fluorophores for fluorescence imaging and targeting ligands for molecular targeting.