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JoVE Journal Developmental Biology
Samlokalisering av Cell Lineage Markörer och tomat Signal
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Samlokalisering av Cell Lineage Markörer och tomat Signal

Article DOI: 10.3791/54982-v • 10:56 min • December 28th, 2016
December 28th, 2016 •
1, 1, 1, 1
1Department of Biomedical Sciences, Texas A&M University College of Dentistry

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Vi utvecklade två uppsättningar spåra kombinationer i ROSA26 tdTomato (ubiquitously uttryckt i alla celler) / Cre (specifikt uttryckt i kondrocyter) möss: en med 2.3Col1a1-GFP (specifik för osteoblaster) och en med immunofluorescens (specifik för benceller). Data visar den direkta omvandlingen av kondrocyter i benceller.

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Utvecklingsbiologi cell härstamning spårning celltransdifferentiering immunofluorescens kondrocyt osteoblast osteocyte
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