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Biochemistry

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Time-løst elektrospray-ionisering Hydrogen-deuterium Veksling massespektrometri for å studere Protein struktur og dynamikk

Article DOI: 10.3791/55464-v • 09:18 min • April 17th, 2017
April 17th, 2017 •
1, 1, 1, 1, 1, 1,2,3
1Department of Chemistry, York University, 2The Centre for Research in Mass Spectrometry, York University, 3The Centre for Research on Biomolecular Interactions, York University

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Konformasjonelle fleksibilitet spiller en kritisk rolle i proteinfunksjon. Heri, beskriver vi bruken av tidsoppløst elektrosprayionisering massespektrometri koplet til hydrogen-deuterium-utveksling for sondering de hurtige strukturelle endringer som driver funksjon i bestilt og uordnede proteiner.

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Biochemistry utgave 122 tidsoppløst elektrosprayionisering massespektrometri hydrogen-deuterium utveksling protein dynamikk egen uordnede proteiner MicroFluidics
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