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Immunology and Infection

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Precisie-cut Mouse Lung Slices Live Pulmonary visualiseren van dendritische cellen

Article DOI: 10.3791/55465-v • 09:33 min • April 5th, 2017
April 5th, 2017 •
1, 1, 1, 1
1Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research, National Institute of Environmental Health Sciences, NIH

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We beschrijven een werkwijze voor het precisie-cut Lung Schijfjes (PCLS) en immunokleuring ze naar de lokalisatie van diverse immuun celsoorten te visualiseren in de longen. Ons protocol kan worden uitgebreid tot de locatie en de functie van vele verschillende celtypen te visualiseren onder verschillende omstandigheden.

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Immunology live cell imaging precisie gesneden long secties long dendritische cellen lokalisatie confocale microscopie
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