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Glucoseopname-meting en respons op insuline stimulatie in

Article DOI: 10.3791/55743-v • 08:03 min • June 25th, 2017
June 25th, 2017 •
1, 1, 1, 2, 3, 1, 1
1CarMeN Laboratory, INSERM U1060, INRA 1397, University of Lyon, 2Department of digestive and bariatric surgery, Obesity Integrated Center, University Hospital of Edouard Herriot, Hospices Civils de Lyon, Lyon 1 University, 3Division of Endocrinology, Diabetes, Hypertension and Nutrition, Department of Clinical Medicine, Faculty of Medicine, University of Geneva

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Bij deze werkwijze worden humane primaire spiercellen in vitro gekweekt om gedifferentieerde myotubes te verkrijgen en de opname van glucose wordt gemeten. Wij bieden een gedetailleerd protocol om de tarieven in basale en insuline gestimuleerde staten te kwantificeren met radioactief gemerkte [3H] 2-deoxy-D-glucose.

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Cellular Biology Skeletspier glucose metabolisme insuline signalering humane myotube insuline gevoeligheid radioactiviteit
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