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Retrograd neuroanatomiska spårning av Phrenic motoriska nervceller hos möss

Article DOI: 10.3791/56758-v • 09:05 min • February 22nd, 2018
February 22nd, 2018 •
1, 1, 1, 2, 2
1URVI, NARILIS, Université de Namur, 2URPhyM, NARILIS, Université de Namur

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Här beskriver vi ett protokoll för att identifiera phrenic motoriska nervceller hos möss efter intrapleural leverans av fluorophore konjugerat kolera toxin subenhet beta. Två tekniker jämförs för att injicera pleural hålighet: transdiaphragmatic kontra transtorakal metoder.

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Neurovetenskap fråga 132 Phrenic motoriska nervceller retrograd märkning intrapleural kolera toxin subenhet beta möss neuroanatomiska tracer
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