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Expressie van fluorescerende Fusion eiwitten in lymfkliertest beenmerg-afgeleide dendritische cellen en macrofagen

Article DOI: 10.3791/58081-v • 09:07 min • October 30th, 2018
October 30th, 2018 •
1,2, 1,2,3, 1,2, 1
1Laboratory of Leukocyte Signalling, Institute of Molecular Genetics of the ASCR, 2Faculty of Science, Charles University, 3Department of Biophysical Chemistry, J. Heyrovsky Institute of Physical Chemistry ASCR

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In dit artikel geven wij dat een gedetailleerd protocol voor de expressie van fluorescerende fusion eiwitten in lymfkliertest beenmerg-afgeleide dendritische cellen en macrofagen. De methode is gebaseerd op de transductie van het beenmerg progenitoren met retrovirale constructies gevolgd door differentiatie in macrofagen en dendritische cellen in vitro.

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Biologie kwestie 140 dendritische cellen macrofagen myeloïde cellen differentiatie lymfkliertest beenmergcellen cytokines M-CSF GM-CSF virale infectie GFP gelabeld eiwit
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