Waiting
登录处理中...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / 利用南方印迹和聚合酶链反应鉴定小鼠胚胎干细胞的同源重组事件…
JoVE Journal
Genetics

需要订阅 JoVE 才能查看此 内容. 登录或开始免费试用。

JoVE Journal Genetics
利用南方印迹和聚合酶链反应鉴定小鼠胚胎干细胞的同源重组事件
Microinjection Method for Anopheles gambiae Embryos
Journal (Genetics)
Microinjection Method for Anopheles gambiae Embryos
Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
Journal (Genetics)
Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
 
Click here for the English version

利用南方印迹和聚合酶链反应鉴定小鼠胚胎干细胞的同源重组事件

Article DOI: 10.3791/58467-v • 08:01 min • November 20th, 2018
November 20th, 2018 •
*1,2, *1, *3, 1, 1, 1, 4, 5, 3, 1
1Lab of Animal Models and Functional Genomics (LAMFG), The Key Laboratory of Animal Vaccine & Protein Engineering, College of Veterinary Medicine, Hunan Agricultural University (HUNAU), 2Department of Pathology, Georgetown University Medical School, 3College of Food Science and Technology, Hunan Agricultural University (HUNAU), 4Lab of Molecular Cardiology (LMC), National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH), 5Transgenic Core, National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH)
* These authors contributed equally

章节

总结概括
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

在这里, 我们提出了一个详细的协议, 以确定同源重组事件发生在小鼠胚胎干细胞使用南方印迹和/或 pcr。利用传统的胚胎干细胞同源重组介导靶向技术生成的非肌肉肌球蛋白 ii 基因替代小鼠模型就是例证。

Tags

遗传学 第141期 同源重组 基因靶向 南方印迹 pcr 小鼠 胚胎干细胞 探针 基因替代 敲除/敲入 myh9 基因 基因组 dna
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter