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平滑肌细胞近系追踪小鼠晚期动脉粥样硬化病变细胞成分的定量分析
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医学
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JoVE 杂志 医学
Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice
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平滑肌细胞近系追踪小鼠晚期动脉粥样硬化病变细胞成分的定量分析

DOI:
10.3791/59139-v

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09:06 min

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February 20, 2019

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, , ,
1Heart, Lung, Blood and Vascular Medicine Institute,University of Pittsburgh, 2Robert M. Berne Cardiovascular Research Center,University of Virginia, 3Department of Biochemistry and Molecular Genetics,University of Virginia, 4Division of Cardiology,University of Pittsburgh School of Medicine

Chapters

  • 00:04Title
  • 00:36Brachiocephalic Artery (BCA) Harvest Preparation
  • 01:56BCA Exposure and Harvest
  • 03:05Immunofluorescent Staining
  • 05:04Confocal Microscopy and Single Cell Counting
  • 07:00Results: Representative Characterization of the Effects of IL-1beta Inhibition on the Cellular Composition of Artherosclerotic Lesions
  • 08:33Conclusion

Summary

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我们提出了一个标准化的方案来表征晚期小鼠动脉粥样硬化病变的细胞组成, 包括动物解剖、组织包埋、切片、染色和腕头动脉分析的系统方法从动脉粥样硬化平滑肌细胞谱系追踪小鼠。

Tags

Quantitative AnalysisCellular CompositionAdvanced Atherosclerotic LesionsSmooth Muscle Cell Lineage-tracingImmunofluorescent StainingPhenotypic CharacterizationQualitative And Quantitative AnalysesCell TypesBrachiocephalic Artery HarvestGravity Perfusion SystemParaformaldehyde FixationCarotid ArterySubclavian ArteryAortic Arch

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