Waiting
登录处理中...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Chemistry / NMR-based Activity Assays for Determining…
JoVE Journal
Chemistry

需要订阅 JoVE 才能查看此 内容. 登录或开始免费试用。

JoVE Journal Chemistry
NMR-based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases
Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
Journal (Chemistry)
Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group
Journal (Chemistry)
An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group
 
Click here for the English version

NMR-based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases

Article DOI: 10.3791/59928-v • 10:24 min • June 30th, 2019
June 30th, 2019 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1,2, 1,3
1Department of Chemistry, Adelphi University, 2Department of Chemistry, Washington University in St. Louis, 3Department of Chemistry, Boston University

章节

总结概括
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

NMR-basierte Aktivitätsassays wurden entwickelt, um Inhibitoren von zwei Nukleosid-Ribohydrolase-Enzymen zu identifizieren und zu charakterisieren. Es werden Protokolle für anfängliche zusammengesetzte Assays mit 500 m und 250 M, Dosis-Wirkungs-Assays zur Bestimmung der IC-50-Werte, Waschmittel-Gegensieb-Assays, Jump-Dilution-Zähler-Screen-Assays und Assays in E. coli-Vollzellen bereitgestellt.

Tags

Chemie Ausgabe 148 Waschmittelzähler Enzymaktivitätstest IC 50-Wert Sprungverdünnung 1H NMR 19F NMR Ganze Zelle NMR Nukleosidribohydrolase
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter