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JoVE Journal Behavior
Embryomikroinjeksjon og knockout mutant identifikasjon av CRISPR/Cas9 Genom-redigert Helicoverpa Armigera (Hübner)
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Embryomikroinjeksjon og knockout mutant identifikasjon av CRISPR/Cas9 Genom-redigert Helicoverpa Armigera (Hübner)

Article DOI: 10.3791/62068-v • 06:37 min • July 1st, 2021
July 1st, 2021 •
1,2, 2, 1, 1, 1, 2,3
1School of Chemical and Environmental Engineering, China University of Mining and Technology, 2State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 3Guangdong Laboratory of Lingnan Modern Agriculture, Shenzhen; Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences

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Presentert her er en protokoll av Helicoverpa armigera (Hübner) embryo mikroinjeksjon og knockout mutant identifikasjon opprettet av CRISPR / Cas9 genom redigering. Mutante insekter muliggjør videre forskning på genfunksjon og interaksjon mellom ulike gener in vivo.

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Atferd Utgave 173 Helicoverpa armigera embryomikroinjeksjon gen knockouts CRISPR Cas9
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