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JoVE Journal Neuroscience
Mätning av egenskaper hos det periodiska skelettet i Axons ursprungliga segment med hjälp av 3D-strukturerad belysningsmikroskopi (3D-SIM)
Investigation of Spatial Interaction Between Astrocytes and Neurons in Cleared Brains
Journal (Neuroscience)
Investigation of Spatial Interaction Between Astrocytes and Neurons in Cleared Brains
Ex-Vivo Brain Slices Preparation from Mouse Brain Tissue for Studying Hippocampal-Cortical and Olfactory Bulb Circuits
Journal (Neuroscience)
Ex-Vivo Brain Slices Preparation from Mouse Brain Tissue for Studying Hippocampal-Cortical and Olfactory Bulb Circuits
 
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Mätning av egenskaper hos det periodiska skelettet i Axons ursprungliga segment med hjälp av 3D-strukturerad belysningsmikroskopi (3D-SIM)

Article DOI: 10.3791/63327-v • 07:40 min • February 11th, 2022
February 11th, 2022 •
1, 2, 1,3,4
1Minerva Foundation Institute for Medical Research, 2Department of Computer Science, Aalto University School of Science, 3HiLIFE - Neuroscience Center, University of Helsinki, 4HiLIFE - Institute of Biotechnology, University of Helsinki

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Detta protokoll beskriver en metod för att visualisera och mäta aktin ringar och andra komponenter i membranet periodiska skelettet i axon inledande segmentet med hjälp av odlade råtta hippocampal nervceller och 3D-strukturerad belysning mikroskopi (3D-SIM).

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Neurovetenskap nummer 180
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