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De novo Identifikation af aktivt oversatte åbne læserammer med ribosomprofileringsdata

Article DOI: 10.3791/63366-v • 08:23 min • February 18th, 2022
February 18th, 2022 •
*1, *2,3, 2,3, 1
1School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center, 2MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, 3Joint Graduate Program of Peking-Tsinghua-National Institute of Biological Science
* These authors contributed equally

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Oversættelse af ribosomer afkoder tre nukleotider pr. Codon til peptider. Deres bevægelse langs mRNA, fanget ved ribosomprofilering, producerer fodsporene, der udviser karakteristisk tripletperioditet. Denne protokol beskriver, hvordan du bruger RiboCode til at dechiffrere denne fremtrædende funktion fra ribosomprofileringsdata for at identificere aktivt oversatte åbne læserammer på hele transkriptomniveau.

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Biologi Udgave 180 Ribosomprofilering åben læseramme mRNA-translation mikropeptid uORF dORF
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