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Kvantitativ bestemmelse af de novo fedtsyresyntese i brunt fedtvæv under anvendelse af deuteriumoxid

Article DOI: 10.3791/64219-v • 07:34 min • May 12th, 2023
May 12th, 2023 •
*1, *2, 1, 2,3,4
1UCD Conway Institute and UCD Institute of Food and Health, School of Agriculture and Food Science, University College Dublin, 2Division of Endocrinology, Cincinnati Children’s Hospital Medical Center, Cincinnati, 3Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati, 4Department of Pediatrics, University of Cincinnati College of Medicine
* These authors contributed equally

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Her præsenterer vi en billig kvantitativ metode, der anvender deuteriumoxid og gaskromatografi massespektrometri (GCMS) til analyse af total fedtsyre de novo lipogenese i brunt fedtvæv in vivo.

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Biologi udgave 195 Brunt fedtvæv Deuteriumoxid Metabolisk vej Funktionelle roller Helkropsmetabolisk homeostase Fysiologiske processer Patologiske processer Bortskaffelse af glukose Funktionel vurdering Detaljerede protokoller Kvantitativ metode Gaskromatografi massespektrometri (GCMS) Total fedtsyre de novo-syntese kulstofkilde prøveforberedelse nedstrømsberegninger brunt fedt metabolisk homeostase
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