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JoVE Journal Bioengineering
Medição in vivo de neuropeptídeos por imunoprobe capacitivo no coração suíno
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Medição in vivo de neuropeptídeos por imunoprobe capacitivo no coração suíno

Article DOI: 10.3791/63926-v • 08:20 min • May 19th, 2022
May 19th, 2022 •
1, 1, 2,3, 1
1Department of Physiology and Biophysics, Case Western Reserve University, 2University of California Los Angeles (UCLA) Cardiac Arrhythmia Center, David Geffen School of Medicine, 3UCLA Neurocardiology Research Program of Excellence, UCLA

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Métodos imunoquímicos estabelecidos para medir transmissores de peptídeos in vivo dependem de microdiálise ou desenho de fluido a granel para obter a amostra para análise offline. No entanto, estes sofrem de limitações espessotemporais. O presente protocolo descreve a fabricação e aplicação de um biosensor de imunoprobe capacitivo que supera as limitações das técnicas existentes.

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Bioengenharia Edição 183
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