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Isolation of Retinal Pigment Epithelial Cells from Guinea Pig Eyes
JoVE 杂志
生物学
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JoVE 杂志 生物学
Isolation of Retinal Pigment Epithelial Cells from Guinea Pig Eyes

Isolation of Retinal Pigment Epithelial Cells from Guinea Pig Eyes

DOI:
10.3791/64837-v

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05:06 min

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May 09, 2023

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, ,
1Herbert Wertheim School Optometry and Vision Science,University of California, Berkeley, 2Department of Ophthalmology,Osaka University Graduate School of Medicine, 3Department of Ophthalmology, Tokyo Medical Center,National Hospital Organization, 4Department of Optometry and Vision Science, School of Optometry,University of Alabama at Birmingham

Chapters

  • 00:05Introduction
  • 00:59Isolating the Ocular Posterior Eye Cup and the RPE/Choroid/Sclera Complex
  • 01:57Isolation of the RPE from the Choroid
  • 03:47Results: Assessing the Quality of the Isolated RPE Samples
  • 04:29Conclusion

Summary

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We describe a simple and efficient method for isolating cells of the retinal pigment epithelium (RPE) cells from the eyes of young pigmented guinea pigs. This procedure allows for follow-up molecular biology studies on the isolated RPE, including gene expression analyses.

Tags

Retinal Pigment Epithelial CellsRPE IsolationGuinea Pig EyesRetinal HealthRetinal DiseaseMyopia ModelBiological Molecular StudiesRPE AnalysisEnucleationPBSScleraCorneaIrisCiliary BodyCrystalline LensZonule Of ZinnRetinaChoroidScleraPBS RinseRPE DetachmentChoroidCentrifugation

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