Journal
/
/
Isolation of Retinal Pigment Epithelial Cells from Guinea Pig Eyes
JoVE 杂志
生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物学
Isolation of Retinal Pigment Epithelial Cells from Guinea Pig Eyes
DOI:

05:06 min

May 09, 2023

, ,

Chapters

  • 00:05Introduction
  • 00:59Isolating the Ocular Posterior Eye Cup and the RPE/Choroid/Sclera Complex
  • 01:57Isolation of the RPE from the Choroid
  • 03:47Results: Assessing the Quality of the Isolated RPE Samples
  • 04:29Conclusion

Summary

自动翻译

We describe a simple and efficient method for isolating cells of the retinal pigment epithelium (RPE) cells from the eyes of young pigmented guinea pigs. This procedure allows for follow-up molecular biology studies on the isolated RPE, including gene expression analyses.

Related Videos

Read Article